Baliakandi and Rajbari Sadar upazilas on the north, Boalmari and Mohammadpur (Magura) upazilas on the south, Faridpur Sadar upazila on the east, Magura Sadar and Sreepur (Magura) upazilas on the west.

Madhukhali thana was turned into an upazila in 1983. The upazila consists of 9 union parishads and 240 villages.

Madhukhali Upazila is located in Faridpur District. Upazilla(s)/Thana(s) in Faridpur are Alfadanga Upazila, Bhanga Upazila, Boalmari Upazila, Char Bhadrasan Upazila, Faridpur Sadar Upazila, Madhukhali Upazila, Nagarkanda Upazila, Sadarpur Upazila.

Seventy feet high wall at Mathurapur (c 16th century), Kachari (zamindar's revenue office) and Dighi (pond) of Mukunda Ray (Sitaram) at Busna.